Construct: shRNA TRCN0000222081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003262.2-1267s1c2, TRCN0000062872, NM_003262.2-1267s1c1
DNA Barcode:
GAAATGAGAGTAGGTGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC62 (7095)
Gene Description:
SEC62 homolog, preprotein translocation factor
Transcript:
RefSeq NM_003262.2 (NON-CURRENT)
Match location:
Position 1267 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATGAGAGTAGGTGTTTAT
Hairpin Sequence:
5'-CCGG-GAAATGAGAGTAGGTGTTTAT-CTCGAG-ATAAACACCTACTCTCATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATGAGAGTAGGTGTTTATCTCGAGATAAACACCTACTCTCATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATGAGAGTAGGTGTTTATCTCGAGATAAACACCTACTCTCATTTC-3'

Other clones with same target sequence:

  1. TRCN0000289833
  2. TRCN0000191679
  3. TRCN0000293119