Construct: shRNA TRCN0000222084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004051.1-1693s1c2, TRCN0000062876, NM_001004051.1-1693s1c1
DNA Barcode:
GAGTCTGAAGATGAGTTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPRASP2 (114928)
Gene Description:
G protein-coupled receptor associated sorting protein 2
Transcript:
RefSeq NM_001004051.1 (NON-CURRENT)
Match location:
Position 1693 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCTGAAGATGAGTTCTAT
Hairpin Sequence:
5'-CCGG-GAGTCTGAAGATGAGTTCTAT-CTCGAG-ATAGAACTCATCTTCAGACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCTGAAGATGAGTTCTATCTCGAGATAGAACTCATCTTCAGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCTGAAGATGAGTTCTATCTCGAGATAGAACTCATCTTCAGACTC-3'

Other clones with same target sequence:

(none)