Construct: shRNA TRCN0000222113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003152.2-2558s1c2, TRCN0000019306, NM_003152.2-2558s1c1
DNA Barcode:
CCTGTGGAACCTGAAACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT5A (6776)
Gene Description:
signal transducer and activator of transcription 5A
Transcript:
RefSeq NM_003152.2 (NON-CURRENT)
Match location:
Position 2558 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGGAACCTGAAACCATT
Hairpin Sequence:
5'-CCGG-CCTGTGGAACCTGAAACCATT-CTCGAG-AATGGTTTCAGGTTCCACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGGAACCTGAAACCATTCTCGAGAATGGTTTCAGGTTCCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGGAACCTGAAACCATTCTCGAGAATGGTTTCAGGTTCCACAGG-3'

Other clones with same target sequence:

(none)