Construct: shRNA TRCN0000222116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376578.1-605s1c2, TRCN0000019310, XM_376578.1-605s1c1
DNA Barcode:
CCTGTAGTGATTCTGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHF14 (9678)
Gene Description:
PHD finger protein 14
Transcript:
RefSeq XM_376578.1 (NON-CURRENT)
Match location:
Position 605 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTAGTGATTCTGAAGAAA
Hairpin Sequence:
5'-CCGG-CCTGTAGTGATTCTGAAGAAA-CTCGAG-TTTCTTCAGAATCACTACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTAGTGATTCTGAAGAAACTCGAGTTTCTTCAGAATCACTACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTAGTGATTCTGAAGAAACTCGAGTTTCTTCAGAATCACTACAGG-3'

Other clones with same target sequence:

  1. TRCN0000286001