Construct: shRNA TRCN0000222170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000901.1-1386s1c2, TRCN0000019366, NM_000901.1-1386s1c1
DNA Barcode:
CCAGCTTAATATTGTCCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR3C2 (4306)
Gene Description:
nuclear receptor subfamily 3 group C member 2
Transcript:
RefSeq NM_000901.1 (NON-CURRENT)
Match location:
Position 1386 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTTAATATTGTCCAGTA
Hairpin Sequence:
5'-CCGG-CCAGCTTAATATTGTCCAGTA-CTCGAG-TACTGGACAATATTAAGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTTAATATTGTCCAGTACTCGAGTACTGGACAATATTAAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTTAATATTGTCCAGTACTCGAGTACTGGACAATATTAAGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000336626