Construct: shRNA TRCN0000222179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015642.1-2171s1c2, TRCN0000019375, NM_015642.1-2171s1c1
DNA Barcode:
CCAGTGTAGTATCTGCAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZBTB20 (26137)
Gene Description:
zinc finger and BTB domain containing 20
Transcript:
RefSeq NM_015642.1 (NON-CURRENT)
Match location:
Position 2171 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGTAGTATCTGCAACAA
Hairpin Sequence:
5'-CCGG-CCAGTGTAGTATCTGCAACAA-CTCGAG-TTGTTGCAGATACTACACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGTAGTATCTGCAACAACTCGAGTTGTTGCAGATACTACACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGTAGTATCTGCAACAACTCGAGTTGTTGCAGATACTACACTGG-3'

Other clones with same target sequence:

(none)