Construct: shRNA TRCN0000222183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006696.3-2594s1c2, TRCN0000019379, NM_006696.3-2594s1c1
DNA Barcode:
CGTGACATTATGCTGATGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD8 (10902)
Gene Description:
bromodomain containing 8
Transcript:
RefSeq NM_006696.3 (NON-CURRENT)
Match location:
Position 2594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGACATTATGCTGATGTTT
Hairpin Sequence:
5'-CCGG-CGTGACATTATGCTGATGTTT-CTCGAG-AAACATCAGCATAATGTCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGACATTATGCTGATGTTTCTCGAGAAACATCAGCATAATGTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGACATTATGCTGATGTTTCTCGAGAAACATCAGCATAATGTCACG-3'

Other clones with same target sequence:

(none)