Construct: shRNA TRCN0000222187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006696.3-1481s1c2, TRCN0000019383, NM_006696.3-1481s1c1
DNA Barcode:
GCTGTGTCTTACACAGGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD8 (10902)
Gene Description:
bromodomain containing 8
Transcript:
RefSeq NM_006696.3 (NON-CURRENT)
Match location:
Position 1481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTCTTACACAGGTGAA
Hairpin Sequence:
5'-CCGG-GCTGTGTCTTACACAGGTGAA-CTCGAG-TTCACCTGTGTAAGACACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTCTTACACAGGTGAACTCGAGTTCACCTGTGTAAGACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTCTTACACAGGTGAACTCGAGTTCACCTGTGTAAGACACAGC-3'

Other clones with same target sequence:

(none)