Construct: shRNA TRCN0000222204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007788.2-1210s1c2, TRCN0000026753, NM_007788.2-1210s1c1
DNA Barcode:
GCCAATATGATGTCAGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csnk2a1 (12995)
Gene Description:
casein kinase 2, alpha 1 polypeptide
Transcript:
RefSeq NM_007788.2 (NON-CURRENT)
Match location:
Position 1210 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATATGATGTCAGGGATT
Hairpin Sequence:
5'-CCGG-GCCAATATGATGTCAGGGATT-CTCGAG-AATCCCTGACATCATATTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATATGATGTCAGGGATTCTCGAGAATCCCTGACATCATATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATATGATGTCAGGGATTCTCGAGAATCCCTGACATCATATTGGC-3'

Other clones with same target sequence:

(none)