Construct: shRNA TRCN0000222221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019777.2-2455s1c2, TRCN0000026726, NM_019777.2-2455s1c1
DNA Barcode:
CGACTCATCGAACGGTTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ikbke (56489)
Gene Description:
inhibitor of kappaB kinase epsilon
Transcript:
RefSeq NM_019777.2 (NON-CURRENT)
Match location:
Position 2455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTCATCGAACGGTTACAT
Hairpin Sequence:
5'-CCGG-CGACTCATCGAACGGTTACAT-CTCGAG-ATGTAACCGTTCGATGAGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTCATCGAACGGTTACATCTCGAGATGTAACCGTTCGATGAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTCATCGAACGGTTACATCTCGAGATGTAACCGTTCGATGAGTCG-3'

Other clones with same target sequence:

  1. TRCN0000350726