Construct: shRNA TRCN0000222229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144847.1-491s1c2, TRCN0000026763, NM_144847.1-491s1c1
DNA Barcode:
CTGAGAATGTGGTAGAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nrbp2 (223649)
Gene Description:
nuclear receptor binding protein 2
Transcript:
RefSeq NM_144847.1 (CURRENT)
Match location:
Position 491 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGAATGTGGTAGAGGAAA
Hairpin Sequence:
5'-CCGG-CTGAGAATGTGGTAGAGGAAA-CTCGAG-TTTCCTCTACCACATTCTCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGAATGTGGTAGAGGAAACTCGAGTTTCCTCTACCACATTCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGAATGTGGTAGAGGAAACTCGAGTTTCCTCTACCACATTCTCAG-3'

Other clones with same target sequence:

(none)