Construct: shRNA TRCN0000222239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173032.1-1639s1c2, TRCN0000026710, NM_173032.1-1639s1c1
DNA Barcode:
CCTCCACTTATGAGAGGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbck (271981)
Gene Description:
TBC1 domain containing kinase
Transcript:
RefSeq NM_173032.1 (NON-CURRENT)
Match location:
Position 1639 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCACTTATGAGAGGTTTA
Hairpin Sequence:
5'-CCGG-CCTCCACTTATGAGAGGTTTA-CTCGAG-TAAACCTCTCATAAGTGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCACTTATGAGAGGTTTACTCGAGTAAACCTCTCATAAGTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCACTTATGAGAGGTTTACTCGAGTAAACCTCTCATAAGTGGAGG-3'

Other clones with same target sequence:

(none)