Construct: shRNA TRCN0000222260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201370.1-1546s1c2, TRCN0000026728, NM_201370.1-1546s1c1
DNA Barcode:
GCAGAGTCTTTACCCATCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wee2 (381759)
Gene Description:
WEE1 homolog 2 (S. pombe)
Transcript:
RefSeq NM_201370.1 (NON-CURRENT)
Match location:
Position 1546 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTCTTTACCCATCAAT
Hairpin Sequence:
5'-CCGG-GCAGAGTCTTTACCCATCAAT-CTCGAG-ATTGATGGGTAAAGACTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTCTTTACCCATCAATCTCGAGATTGATGGGTAAAGACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTCTTTACCCATCAATCTCGAGATTGATGGGTAAAGACTCTGC-3'

Other clones with same target sequence:

(none)