Construct: shRNA TRCN0000222267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_147201.1-992s1c2, TRCN0000026706, NM_147201.1-992s1c1
DNA Barcode:
GACTCATTACAGAGGGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nrbp1 (192292)
Gene Description:
nuclear receptor binding protein 1
Transcript:
RefSeq NM_147201.1 (NON-CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCATTACAGAGGGAGTTT
Hairpin Sequence:
5'-CCGG-GACTCATTACAGAGGGAGTTT-CTCGAG-AAACTCCCTCTGTAATGAGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCATTACAGAGGGAGTTTCTCGAGAAACTCCCTCTGTAATGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCATTACAGAGGGAGTTTCTCGAGAAACTCCCTCTGTAATGAGTC-3'

Other clones with same target sequence:

  1. TRCN0000276710