Construct: shRNA TRCN0000222271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023209.1-584s1c2, TRCN0000026732, NM_023209.1-584s1c1
DNA Barcode:
GCTATGGAGTATGGAGGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pbk (52033)
Gene Description:
PDZ binding kinase
Transcript:
RefSeq NM_023209.1 (NON-CURRENT)
Match location:
Position 584 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGGAGTATGGAGGTGAA
Hairpin Sequence:
5'-CCGG-GCTATGGAGTATGGAGGTGAA-CTCGAG-TTCACCTCCATACTCCATAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGGAGTATGGAGGTGAACTCGAGTTCACCTCCATACTCCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGGAGTATGGAGGTGAACTCGAGTTCACCTCCATACTCCATAGC-3'

Other clones with same target sequence:

(none)