Construct: shRNA TRCN0000222277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021605.2-294s1c2, TRCN0000026773, NM_021605.2-294s1c1
DNA Barcode:
CGTGGACAATTTAGTGAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek7 (59125)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 7
Transcript:
RefSeq NM_021605.2 (NON-CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGACAATTTAGTGAAGTT
Hairpin Sequence:
5'-CCGG-CGTGGACAATTTAGTGAAGTT-CTCGAG-AACTTCACTAAATTGTCCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGACAATTTAGTGAAGTTCTCGAGAACTTCACTAAATTGTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGACAATTTAGTGAAGTTCTCGAGAACTTCACTAAATTGTCCACG-3'

Other clones with same target sequence:

(none)