Construct: shRNA TRCN0000222305
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_029522.1-781s1c2, TRCN0000028914, NM_029522.1-781s1c1
- DNA Barcode:
- GCGCTCTACAATCTTGGAAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gpsm2 (76123)
- Gene Description:
- G-protein signalling modulator 2 (AGS3-like, C. elegans)
- Transcript:
- RefSeq NM_029522.1 (NON-CURRENT)
- Match location:
- Position 781 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000222305)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGCTCTACAATCTTGGAAAT
- Hairpin Sequence:
-
5'-CCGG-GCGCTCTACAATCTTGGAAAT-CTCGAG-ATTTCCAAGATTGTAGAGCGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGCTCTACAATCTTGGAAATCTCGAGATTTCCAAGATTGTAGAGCGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGCTCTACAATCTTGGAAATCTCGAGATTTCCAAGATTGTAGAGCGC-3'
Other clones with same target sequence:
-
TRCN0000292821
