Construct: shRNA TRCN0000222307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029522.1-592s1c2, TRCN0000028929, NM_029522.1-592s1c1
DNA Barcode:
GCGTTAGAATACCACCACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpsm2 (76123)
Gene Description:
G-protein signalling modulator 2 (AGS3-like, C. elegans)
Transcript:
RefSeq NM_029522.1 (NON-CURRENT)
Match location:
Position 592 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTAGAATACCACCACCAT
Hairpin Sequence:
5'-CCGG-GCGTTAGAATACCACCACCAT-CTCGAG-ATGGTGGTGGTATTCTAACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTAGAATACCACCACCATCTCGAGATGGTGGTGGTATTCTAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTAGAATACCACCACCATCTCGAGATGGTGGTGGTATTCTAACGC-3'

Other clones with same target sequence:

  1. TRCN0000292889