Construct: shRNA TRCN0000222322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010195.1-943s1c2, TRCN0000028912, NM_010195.1-943s1c1
DNA Barcode:
GCCTCGCACATTACTGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lgr5 (14160)
Gene Description:
leucine rich repeat containing G protein coupled receptor 5
Transcript:
RefSeq NM_010195.1 (NON-CURRENT)
Match location:
Position 943 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCGCACATTACTGAATTT
Hairpin Sequence:
5'-CCGG-GCCTCGCACATTACTGAATTT-CTCGAG-AAATTCAGTAATGTGCGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCGCACATTACTGAATTTCTCGAGAAATTCAGTAATGTGCGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCGCACATTACTGAATTTCTCGAGAAATTCAGTAATGTGCGAGGC-3'

Other clones with same target sequence:

(none)