Construct: shRNA TRCN0000222329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010336.1-2548s1c2, TRCN0000028893, NM_010336.1-2548s1c1
DNA Barcode:
GCCAGTATCATTGATGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lpar1 (14745)
Gene Description:
lysophosphatidic acid receptor 1
Transcript:
RefSeq NM_010336.1 (NON-CURRENT)
Match location:
Position 2548 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTATCATTGATGATTTA
Hairpin Sequence:
5'-CCGG-GCCAGTATCATTGATGATTTA-CTCGAG-TAAATCATCAATGATACTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTATCATTGATGATTTACTCGAGTAAATCATCAATGATACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTATCATTGATGATTTACTCGAGTAAATCATCAATGATACTGGC-3'

Other clones with same target sequence:

(none)