Construct: shRNA TRCN0000222331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010336.1-1113s1c2, TRCN0000028920, NM_010336.1-1113s1c1
DNA Barcode:
CGAGTTCAACTCTGCTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lpar1 (14745)
Gene Description:
lysophosphatidic acid receptor 1
Transcript:
RefSeq NM_010336.1 (NON-CURRENT)
Match location:
Position 1113 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTTCAACTCTGCTATGAA
Hairpin Sequence:
5'-CCGG-CGAGTTCAACTCTGCTATGAA-CTCGAG-TTCATAGCAGAGTTGAACTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTTCAACTCTGCTATGAACTCGAGTTCATAGCAGAGTTGAACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTTCAACTCTGCTATGAACTCGAGTTCATAGCAGAGTTGAACTCG-3'

Other clones with same target sequence:

(none)