Construct: shRNA TRCN0000222345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009848.2-152s1c2, TRCN0000028895, NM_009848.2-152s1c1
DNA Barcode:
CCTTGGTTTCACCTCTATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Entpd1 (12495)
Gene Description:
ectonucleoside triphosphate diphosphohydrolase 1
Transcript:
RefSeq NM_009848.2 (NON-CURRENT)
Match location:
Position 152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGTTTCACCTCTATCTT
Hairpin Sequence:
5'-CCGG-CCTTGGTTTCACCTCTATCTT-CTCGAG-AAGATAGAGGTGAAACCAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGTTTCACCTCTATCTTCTCGAGAAGATAGAGGTGAAACCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGTTTCACCTCTATCTTCTCGAGAAGATAGAGGTGAAACCAAGG-3'

Other clones with same target sequence:

(none)