Construct: shRNA TRCN0000222366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178060.2-1637s1c2, TRCN0000027053, NM_178060.2-1637s1c1
DNA Barcode:
GAGGTCTTTGAGGATCAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thra (21833)
Gene Description:
thyroid hormone receptor alpha
Transcript:
RefSeq NM_178060.2 (NON-CURRENT)
Match location:
Position 1637 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGTCTTTGAGGATCAGGAA
Hairpin Sequence:
5'-CCGG-GAGGTCTTTGAGGATCAGGAA-CTCGAG-TTCCTGATCCTCAAAGACCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGTCTTTGAGGATCAGGAACTCGAGTTCCTGATCCTCAAAGACCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGTCTTTGAGGATCAGGAACTCGAGTTCCTGATCCTCAAAGACCTC-3'

Other clones with same target sequence:

(none)