Construct: shRNA TRCN0000222368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_132700.4-678s1c2, TRCN0000027072, XM_132700.4-678s1c1
DNA Barcode:
CCTGAGTGAATCTTTGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nr2c2 (22026)
Gene Description:
nuclear receptor subfamily 2, group C, member 2
Transcript:
RefSeq XM_132700.4 (NON-CURRENT)
Match location:
Position 678 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGTGAATCTTTGAACAA
Hairpin Sequence:
5'-CCGG-CCTGAGTGAATCTTTGAACAA-CTCGAG-TTGTTCAAAGATTCACTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGTGAATCTTTGAACAACTCGAGTTGTTCAAAGATTCACTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGTGAATCTTTGAACAACTCGAGTTGTTCAAAGATTCACTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000346253