Construct: shRNA TRCN0000222375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009380.1-434s1c2, TRCN0000027099, NM_009380.1-434s1c1
DNA Barcode:
GCCCAGTAGCAGCATATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thrb (21834)
Gene Description:
thyroid hormone receptor beta
Transcript:
RefSeq NM_009380.1 (NON-CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTAGCAGCATATATAT
Hairpin Sequence:
5'-CCGG-GCCCAGTAGCAGCATATATAT-CTCGAG-ATATATATGCTGCTACTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTAGCAGCATATATATCTCGAGATATATATGCTGCTACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTAGCAGCATATATATCTCGAGATATATATGCTGCTACTGGGC-3'

Other clones with same target sequence:

(none)