Construct: shRNA TRCN0000222384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009107.2-885s1c2, TRCN0000027077, NM_009107.2-885s1c1
DNA Barcode:
CATCTACACCTGTCGGGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rxrg (20183)
Gene Description:
retinoid X receptor gamma
Transcript:
RefSeq NM_009107.2 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTACACCTGTCGGGATAA
Hairpin Sequence:
5'-CCGG-CATCTACACCTGTCGGGATAA-CTCGAG-TTATCCCGACAGGTGTAGATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTACACCTGTCGGGATAACTCGAGTTATCCCGACAGGTGTAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTACACCTGTCGGGATAACTCGAGTTATCCCGACAGGTGTAGATG-3'

Other clones with same target sequence:

(none)