Construct: shRNA TRCN0000222388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011306.2-829s1c2, TRCN0000027100, NM_011306.2-829s1c1
DNA Barcode:
GTCGTGATAACAAAGACTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rxrb (20182)
Gene Description:
retinoid X receptor beta
Transcript:
RefSeq NM_011306.2 (NON-CURRENT)
Match location:
Position 829 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGTGATAACAAAGACTGTA
Hairpin Sequence:
5'-CCGG-GTCGTGATAACAAAGACTGTA-CTCGAG-TACAGTCTTTGTTATCACGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGTGATAACAAAGACTGTACTCGAGTACAGTCTTTGTTATCACGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGTGATAACAAAGACTGTACTCGAGTACAGTCTTTGTTATCACGAC-3'

Other clones with same target sequence:

  1. TRCN0000339869