Construct: shRNA TRCN0000222393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011305.2-1314s1c2, TRCN0000027086, NM_011305.2-1314s1c1
DNA Barcode:
CCTGTTCAACCCTGACTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rxra (20181)
Gene Description:
retinoid X receptor alpha
Transcript:
RefSeq NM_011305.2 (NON-CURRENT)
Match location:
Position 1314 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTCAACCCTGACTCTAA
Hairpin Sequence:
5'-CCGG-CCTGTTCAACCCTGACTCTAA-CTCGAG-TTAGAGTCAGGGTTGAACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTCAACCCTGACTCTAACTCGAGTTAGAGTCAGGGTTGAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTCAACCCTGACTCTAACTCGAGTTAGAGTCAGGGTTGAACAGG-3'

Other clones with same target sequence:

(none)