Construct: shRNA TRCN0000222424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009504.2-813s1c2, TRCN0000027123, NM_009504.2-813s1c1
DNA Barcode:
CCTGGCTGATCTTGTCAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vdr (22337)
Gene Description:
vitamin D (1,25-dihydroxyvitamin D3) receptor
Transcript:
RefSeq NM_009504.2 (NON-CURRENT)
Match location:
Position 813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCTGATCTTGTCAGTTA
Hairpin Sequence:
5'-CCGG-CCTGGCTGATCTTGTCAGTTA-CTCGAG-TAACTGACAAGATCAGCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCTGATCTTGTCAGTTACTCGAGTAACTGACAAGATCAGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCTGATCTTGTCAGTTACTCGAGTAACTGACAAGATCAGCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000328258