Construct: shRNA TRCN0000222432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011244.2-1011s1c2, TRCN0000027045, NM_011244.2-1011s1c1
DNA Barcode:
GCTCAGCATTGCCGACCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rarg (19411)
Gene Description:
retinoic acid receptor, gamma
Transcript:
RefSeq NM_011244.2 (NON-CURRENT)
Match location:
Position 1011 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGCATTGCCGACCAGAT
Hairpin Sequence:
5'-CCGG-GCTCAGCATTGCCGACCAGAT-CTCGAG-ATCTGGTCGGCAATGCTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGCATTGCCGACCAGATCTCGAGATCTGGTCGGCAATGCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGCATTGCCGACCAGATCTCGAGATCTGGTCGGCAATGCTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000279194