Construct: shRNA TRCN0000222484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009906.2-427s1c2, TRCN0000033110, NM_009906.2-427s1c1
DNA Barcode:
GCTGAGTTTCATCGCTATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpp1 (12751)
Gene Description:
tripeptidyl peptidase I
Transcript:
RefSeq NM_009906.2 (NON-CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGTTTCATCGCTATGTA
Hairpin Sequence:
5'-CCGG-GCTGAGTTTCATCGCTATGTA-CTCGAG-TACATAGCGATGAAACTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGTTTCATCGCTATGTACTCGAGTACATAGCGATGAAACTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGTTTCATCGCTATGTACTCGAGTACATAGCGATGAAACTCAGC-3'

Other clones with same target sequence:

(none)