Construct: shRNA TRCN0000222488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024239.1-1830s1c2, TRCN0000033114, NM_024239.1-1830s1c1
DNA Barcode:
GCCTTCTGTTCCTCTTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stambp (70527)
Gene Description:
STAM binding protein
Transcript:
RefSeq NM_024239.1 (NON-CURRENT)
Match location:
Position 1830 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTGTTCCTCTTGAAAT
Hairpin Sequence:
5'-CCGG-GCCTTCTGTTCCTCTTGAAAT-CTCGAG-ATTTCAAGAGGAACAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTGTTCCTCTTGAAATCTCGAGATTTCAAGAGGAACAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTGTTCCTCTTGAAATCTCGAGATTTCAAGAGGAACAGAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000349144