Construct: shRNA TRCN0000222495
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025468.1-277s1c2, TRCN0000033121, NM_025468.1-277s1c1
- DNA Barcode:
- GATCTGCTGTTCCTCACGAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Sec11c (66286)
- Gene Description:
- SEC11 homolog C, signal peptidase complex subunit
- Transcript:
- RefSeq NM_025468.1 (NON-CURRENT)
- Match location:
- Position 277 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000222495)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATCTGCTGTTCCTCACGAAT
- Hairpin Sequence:
-
5'-CCGG-GATCTGCTGTTCCTCACGAAT-CTCGAG-ATTCGTGAGGAACAGCAGATC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATCTGCTGTTCCTCACGAATCTCGAGATTCGTGAGGAACAGCAGATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATCTGCTGTTCCTCACGAATCTCGAGATTCGTGAGGAACAGCAGATC-3'
Other clones with same target sequence:
(none)
