Construct: shRNA TRCN0000222555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015541.1-2045s1c2
DNA Barcode:
GCAAGGACATCCGGTTTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRIG1 (26018)
Gene Description:
leucine rich repeats and immunoglobulin like domains 1
Transcript:
RefSeq NM_015541.1 (NON-CURRENT)
Match location:
Position 2045 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGACATCCGGTTTACAT
Hairpin Sequence:
5'-CCGG-GCAAGGACATCCGGTTTACAT-CTCGAG-ATGTAAACCGGATGTCCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGACATCCGGTTTACATCTCGAGATGTAAACCGGATGTCCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGACATCCGGTTTACATCTCGAGATGTAAACCGGATGTCCTTGC-3'

Other clones with same target sequence:

(none)