Construct: shRNA TRCN0000222574
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_022350.1-3044s1c2
- DNA Barcode:
- CGCCTGTAATCCCAGCACTTT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ERAP2 (64167)
- Gene Description:
- endoplasmic reticulum aminopeptidase 2
- Transcript:
- RefSeq NM_022350.1 (NON-CURRENT)
- Match location:
- Position 3044 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CGCCTGTAATCCCAGCACTTT
- Hairpin Sequence:
- 5'-CCGG-CGCCTGTAATCCCAGCACTTT-CTCGAG-AAAGTGCTGGGATTACAGGCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCCTGTAATCCCAGCACTTTCTCGAGAAAGTGCTGGGATTACAGGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCCTGTAATCCCAGCACTTTCTCGAGAAAGTGCTGGGATTACAGGCG-3'