Construct: shRNA TRCN0000222575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022826.1-1560s1c2
DNA Barcode:
CCTGGTTCCTTATTCCGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCHF7 (64844)
Gene Description:
membrane associated ring-CH-type finger 7
Transcript:
RefSeq NM_022826.1 (NON-CURRENT)
Match location:
Position 1560 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTTCCTTATTCCGGTTT
Hairpin Sequence:
5'-CCGG-CCTGGTTCCTTATTCCGGTTT-CTCGAG-AAACCGGAATAAGGAACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTTCCTTATTCCGGTTTCTCGAGAAACCGGAATAAGGAACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTTCCTTATTCCGGTTTCTCGAGAAACCGGAATAAGGAACCAGG-3'

Other clones with same target sequence:

(none)