Construct: shRNA TRCN0000222637

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011539.2-1340s1c2
DNA Barcode:
CCGTATCTGGACATGGTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbxas1 (21391)
Gene Description:
thromboxane A synthase 1, platelet
Transcript:
RefSeq NM_011539.2 (NON-CURRENT)
Match location:
Position 1340 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222637)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATCTGGACATGGTGATT
Hairpin Sequence:
5'-CCGG-CCGTATCTGGACATGGTGATT-CTCGAG-AATCACCATGTCCAGATACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATCTGGACATGGTGATTCTCGAGAATCACCATGTCCAGATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATCTGGACATGGTGATTCTCGAGAATCACCATGTCCAGATACGG-3'

Other clones with same target sequence:

(none)