Construct: shRNA TRCN0000222638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011690.2-2265s1c2
DNA Barcode:
GCATTCTTGGATGGACAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vars (22321)
Gene Description:
valyl-tRNA synthetase
Transcript:
RefSeq NM_011690.2 (NON-CURRENT)
Match location:
Position 2265 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCTTGGATGGACAACAT
Hairpin Sequence:
5'-CCGG-GCATTCTTGGATGGACAACAT-CTCGAG-ATGTTGTCCATCCAAGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCTTGGATGGACAACATCTCGAGATGTTGTCCATCCAAGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCTTGGATGGACAACATCTCGAGATGTTGTCCATCCAAGAATGC-3'

Other clones with same target sequence:

(none)