Construct: shRNA TRCN0000222661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025983.2-179s1c2
DNA Barcode:
GAAGACCGAGTTCAAAGCGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp5e (67126)
Gene Description:
ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Transcript:
RefSeq NM_025983.2 (NON-CURRENT)
Match location:
Position 179 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACCGAGTTCAAAGCGAA
Hairpin Sequence:
5'-CCGG-GAAGACCGAGTTCAAAGCGAA-CTCGAG-TTCGCTTTGAACTCGGTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACCGAGTTCAAAGCGAACTCGAGTTCGCTTTGAACTCGGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACCGAGTTCAAAGCGAACTCGAGTTCGCTTTGAACTCGGTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000334712