Construct: shRNA TRCN0000222688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173740.1-629s1c2
DNA Barcode:
CGGATATTCTCAGTCACCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Maoa (17161)
Gene Description:
monoamine oxidase A
Transcript:
RefSeq NM_173740.1 (NON-CURRENT)
Match location:
Position 629 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATATTCTCAGTCACCAAT
Hairpin Sequence:
5'-CCGG-CGGATATTCTCAGTCACCAAT-CTCGAG-ATTGGTGACTGAGAATATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATATTCTCAGTCACCAATCTCGAGATTGGTGACTGAGAATATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATATTCTCAGTCACCAATCTCGAGATTGGTGACTGAGAATATCCG-3'

Other clones with same target sequence:

  1. TRCN0000327502