Construct: shRNA TRCN0000222697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000026.1-1535s1c2
DNA Barcode:
CCTCGAGAATTGTTACCTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADSL (158)
Gene Description:
adenylosuccinate lyase
Transcript:
RefSeq NM_000026.1 (NON-CURRENT)
Match location:
Position 1535 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGAGAATTGTTACCTTAA
Hairpin Sequence:
5'-CCGG-CCTCGAGAATTGTTACCTTAA-CTCGAG-TTAAGGTAACAATTCTCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGAGAATTGTTACCTTAACTCGAGTTAAGGTAACAATTCTCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGAGAATTGTTACCTTAACTCGAGTTAAGGTAACAATTCTCGAGG-3'

Other clones with same target sequence:

(none)