Construct: shRNA TRCN0000222755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053186.1-215s1c2
DNA Barcode:
CTCAAAGACTTGGCTATCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnnm3 (94218)
Gene Description:
cyclin M3
Transcript:
RefSeq NM_053186.1 (NON-CURRENT)
Match location:
Position 215 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAAAGACTTGGCTATCGTT
Hairpin Sequence:
5'-CCGG-CTCAAAGACTTGGCTATCGTT-CTCGAG-AACGATAGCCAAGTCTTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAAAGACTTGGCTATCGTTCTCGAGAACGATAGCCAAGTCTTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAAAGACTTGGCTATCGTTCTCGAGAACGATAGCCAAGTCTTTGAG-3'

Other clones with same target sequence:

(none)