Construct: shRNA TRCN0000226135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033197.2-1165s21c1
DNA Barcode:
TCTGGCGAGGGCTGCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
AU015228 (99169)
Gene Description:
expressed sequence AU015228
Transcript:
RefSeq NM_001033197.2 (CURRENT)
Match location:
Position 1165 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGGCGAGGGCTGCTAATAA
Hairpin Sequence:
5'-CCGG-TCTGGCGAGGGCTGCTAATAA-CTCGAG-TTATTAGCAGCCCTCGCCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGGCGAGGGCTGCTAATAACTCGAGTTATTAGCAGCCCTCGCCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGGCGAGGGCTGCTAATAACTCGAGTTATTAGCAGCCCTCGCCAGA-3'

Other clones with same target sequence:

(none)