Construct: shRNA TRCN0000226221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016891.3-2063s21c1
DNA Barcode:
GGTTGTTCCTGCCCATATTGG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r1a (51792)
Gene Description:
protein phosphatase 2, regulatory subunit A, alpha
Transcript:
RefSeq NM_016891.3 (CURRENT)
Match location:
Position 2063 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTGTTCCTGCCCATATTGG
Hairpin Sequence:
5'-CCGG-GGTTGTTCCTGCCCATATTGG-CTCGAG-CCAATATGGGCAGGAACAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTGTTCCTGCCCATATTGGCTCGAGCCAATATGGGCAGGAACAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTGTTCCTGCCCATATTGGCTCGAGCCAATATGGGCAGGAACAACC-3'

Other clones with same target sequence:

(none)