Construct: shRNA TRCN0000226381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009525.3-930s21c1
DNA Barcode:
ACCGCTTTGCCAAGGAGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnt5b (22419)
Gene Description:
wingless-type MMTV integration site family, member 5B
Transcript:
RefSeq NM_009525.3 (CURRENT)
Match location:
Position 930 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGCTTTGCCAAGGAGTTTG
Hairpin Sequence:
5'-CCGG-ACCGCTTTGCCAAGGAGTTTG-CTCGAG-CAAACTCCTTGGCAAAGCGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGCTTTGCCAAGGAGTTTGCTCGAGCAAACTCCTTGGCAAAGCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGCTTTGCCAAGGAGTTTGCTCGAGCAAACTCCTTGGCAAAGCGGT-3'

Other clones with same target sequence:

(none)