Construct: shRNA TRCN0000230799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024751.2-3570s21c1
DNA Barcode:
TGATCATCACTATGGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSTCD (79807)
Gene Description:
glutathione S-transferase C-terminal domain containing
Transcript:
RefSeq NM_024751.2 (NON-CURRENT)
Match location:
Position 3570 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCATCACTATGGATTAAA
Hairpin Sequence:
5'-CCGG-TGATCATCACTATGGATTAAA-CTCGAG-TTTAATCCATAGTGATGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCATCACTATGGATTAAACTCGAGTTTAATCCATAGTGATGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCATCACTATGGATTAAACTCGAGTTTAATCCATAGTGATGATCA-3'

Other clones with same target sequence:

(none)