Construct: shRNA TRCN0000231053

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000141.4-3214s21c1
DNA Barcode:
ATATGGATCAGAGGAGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGFR2 (2263)
Gene Description:
fibroblast growth factor receptor 2
Transcript:
RefSeq NM_000141.4 (CURRENT)
Match location:
Position 3214 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231053)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATGGATCAGAGGAGTAAAT
Hairpin Sequence:
5'-CCGG-ATATGGATCAGAGGAGTAAAT-CTCGAG-ATTTACTCCTCTGATCCATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATGGATCAGAGGAGTAAATCTCGAGATTTACTCCTCTGATCCATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATGGATCAGAGGAGTAAATCTCGAGATTTACTCCTCTGATCCATAT-3'

Other clones with same target sequence:

  1. TRCN0000321949