Construct: shRNA TRCN0000231148
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005544.2-1429s21c1
- DNA Barcode:
- GGAGGAGCTAAGCAACTATAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IRS1 (3667)
- Gene Description:
- insulin receptor substrate 1
- Transcript:
- RefSeq NM_005544.2 (CURRENT)
- Match location:
- Position 1429 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000231148)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GGAGGAGCTAAGCAACTATAT
- Hairpin Sequence:
-
5'-CCGG-GGAGGAGCTAAGCAACTATAT-CTCGAG-ATATAGTTGCTTAGCTCCTCC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGGAGGAGCTAAGCAACTATATCTCGAGATATAGTTGCTTAGCTCCTCCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGGAGGAGCTAAGCAACTATATCTCGAGATATAGTTGCTTAGCTCCTCC-3'
Other clones with same target sequence:
(none)
