Construct: shRNA TRCN0000231150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005544.2-3711s21c1
DNA Barcode:
GCCGCTCAAGTGAGGATTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRS1 (3667)
Gene Description:
insulin receptor substrate 1
Transcript:
RefSeq NM_005544.2 (CURRENT)
Match location:
Position 3711 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCTCAAGTGAGGATTTAA
Hairpin Sequence:
5'-CCGG-GCCGCTCAAGTGAGGATTTAA-CTCGAG-TTAAATCCTCACTTGAGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCTCAAGTGAGGATTTAACTCGAGTTAAATCCTCACTTGAGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCTCAAGTGAGGATTTAACTCGAGTTAAATCCTCACTTGAGCGGC-3'

Other clones with same target sequence:

  1. TRCN0000039909