Construct: shRNA TRCN0000231244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009754.3-433s21c1
DNA Barcode:
GACGAGTTCAACGAAACTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcl2l11 (12125)
Gene Description:
BCL2-like 11 (apoptosis facilitator)
Transcript:
RefSeq NM_009754.3 (CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGAGTTCAACGAAACTTAC
Hairpin Sequence:
5'-CCGG-GACGAGTTCAACGAAACTTAC-CTCGAG-GTAAGTTTCGTTGAACTCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGAGTTCAACGAAACTTACCTCGAGGTAAGTTTCGTTGAACTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGAGTTCAACGAAACTTACCTCGAGGTAAGTTTCGTTGAACTCGTC-3'

Other clones with same target sequence:

(none)